How to measure microparticles in prevention and rehabilitation

5 November 2018

Organised by:

Cell-derived sub-micron vesicles, the so-called “microparticles” have come into the focus of interest as a new class of circulating mediators, potentially able to serve as biomarkers for the activation state of specific cells or organs, such as the vascular endothelium, and harbouring prognostic potential. In cardiovascular rehabilitation, microparticle monitoring might support the progress towards “personal” rehabilitation programmes.

References

A number of method papers have described the assessment of microparticles, including:

1. Robert S, Lacroix R, Poncelet P, Harhouri K, Bouriche T, Judicone C, Wischhusen J, Arnaud L, Dignat-George F. High-sensitivity flow cytometry provides access to standardized measurement of small-size microparticles--brief report. Arterioscler Thromb Vasc Biol. 2012;32(4):1054-8.

2. Shah MD, Bergeron AL, Dong JF, López JA. Flow cytometric measurement of microparticles: pitfalls and protocol modifications. Platelets. 2008;19(5):365-72.

3. Lacroix R, Robert S, Poncelet P, Dignat-George F. Overcoming limitations of microparticle measurement by flow cytometry. Semin Thromb Hemost. 2010;36(8):807-18.

4. Kretzschmar J, Babbitt DM, Diaz KM, Feairheller DL, Sturgeon KM, Perkins AM, Veerabhadrappa P, Williamson ST, Ling C, Lee H, Grimm H, Thakkar SR, Crabbe DL, Kashem MA, Brown MD. A standardized exercise intervention differentially affects premenopausal and postmenopausal African-American women.
Menopause. 2013 Nov 4. [Epub ahead of print]

Further reading on the role of microparticles in inflammation, cardiovascular disease and rehabilitation:

5. Théry C, Ostrowski M, Segura E. Membrane vesicles as conveyors of immune responses. Nat Rev Immunol. 2009 Aug;9(8):581-93.

6. Loyer X, Vion AC, Tedgui A, Boulanger CM. Microvesicles as cell-cell messengers in cardiovascular diseases. Circ Res. 2014 Jan 17;114(2):345-53.

7. Augustine D, Ayers LV, Lima E, Newton L, Lewandowski AJ, Davis EF, Ferry B, Leeson P. Dynamic release and clearance of circulating microparticles during cardiac stress. Circ Res. 2014 Jan 3;114(1):109-13.

8. Angelillo-Scherrer A. Leukocyte-derived microparticles in vascular homeostasis. Circ Res. 2012 Jan 20;110(2):356-69. doi: 10.1161/CIRCRESAHA.110.233403.

9. Boyle LJ, Credeur DP, Jenkins NT, Padilla J, Leidy HJ, Thyfault JP, Fadel PJ. Impact of reduced daily physical activity on conduit artery flow-mediated dilation and circulating endothelial microparticles. J Appl Physiol (1985). 2013;115(10):1519-25.