OR45. Improving the migratory function of endothelial progenitor cells under high glucose conditions via engineering with human umbilical cord blood mesenchymal stem cell-derived secretome

Human umbilical cord blood mesenchymal stem cells (hUCB-MSC) secreted a group of paracrine factors known as secretome. It has the ability to promote neovascularization and angiogenesis, and it is thought to be capable of modulating EPC function. The capability of hUCB-MSCs-derived secretome in enhancing EPC migration under high glucose condition, which is expected to mimic the metabolic disturbance that occurs in patients with type 2 diabetes mellitus, has been not yet established. This study aims to elucidate the effect of Human Umbilical Cord Blood Mesenchymal Stem Cell (hUCB-MSCs)-derived secretome on the EPCs migration under high glucose conditions.

EPCs were isolated from peripheral blood samples of chronic coronary syndrome patient and cultured for three days. Cultured EPCs were incubated with high glucose for five days and were treated with secretome at various concentration. The migration of EPCs was assessed using the Transwell migration assay. hUCB-MSC-derived secretome induced the migration capability of EPC with at least 20% concentration of secretome, this effect was more potent under high glucose conditions with the least concentration of secretome was 10% (p < 0.001). The migratory function of EPCs was intact on short term exposure of high glucose.

Our finding suggest that hUCB-MSCs-derived secretome may enhance migratory function of EPCs under high glucose conditions. hUCB-MSCs-derived secretome may serve as a promising cell-free regenerative therapy.