Leonardo Sacconi received the Master degree in Physics in 2001 at the University of Florence and then he moved to Trento, where he obtained the Ph.D. in Physics in 2004. He spent one year as a visiting scientist in the Watt Webb laboratory at Cornell University. There he developed an imaging method to optically record neuronal action potentials using non-linear microscopy. When he returned to Italy, to the laboratory of Francesco Pavone at European Laboratory for Nonlinear Spectroscopy, he started new research projects focused on the in vivo functional imaging of brain by non-linear microscopy. He is now a researcher at the National Institute of Optics in Florence and group leader of the Cardiac Imaging group where he is developing innovative imaging methodologies to increase our understanding of cardiac physiology. In 2020 Leonardo joined the Institute for Experimental Cardiovascular Medicine in Freiburg as a visiting scientist.