AIMS: To investigate GQ-11 effects in I/R-related damage.
METHODS: Male Wistar rats were pre-treated by gavage with Vehicle (NaCl 0.9% Tween 0.25%), commercial PPARy agonist (10 mg/kg) or GQ-11 (10 mg/kg) for 7 days and submitted to supraceliac aorta clamping for 30 minutes and 3 hours reperfusion. Positron Emission Tomography (PET) was performed observing 18F-FDG uptake in liver and bowel area in a microPET (Albira, Carestream Health) for 15 minutes imaging acquisition. Organs and blood were analyzed by RT-PCR and Western Blot.
RESULTS: 18F-FDG uptake was decreased - qualitative and quantitative (SUVmax) - in liver and bowel of animals treated with GQ-11 when compared to animals treated with commercial PPARy agonist or controls. The treatment also modulated IL6, IL1b, IL10, TNFa, TGFb, CCL2 and Mt1 indicating inflammation/oxidative stress modulation to I/R damage protection.
CONCLUSION: Regulation of both inflammation and oxidative stress seems to be an important target in the search for I/R management, suggesting that PPAR agonists - including GQ-11 - might be important mediators in these conditions.