Purpose: To evaluate gut microbiota composition in CAD population. To compare the composition of the intestinal microbiome of CAD patients with HF-REF and those who have ejection fraction (EF) at least of 40%.
Methods: The study included stool microbiome of 89 CAD patients, aged 64.3±7.4 years (72 males) who were hospitalized 12-18 months prior recruitment due to an acute coronary syndrome (n=57) or percutaneous coronary intervention (n=32). All patients underwent detailed assessment of health status, including echocardiography with biplane ejection fraction (EF) assessment. Based on EF, HF-REF subgroup was distinguished (n=12).
Stool samples were collected in Stool Tubes with DNA stabilizer. DNA was isolated with the use of the PSP-Spin Stool-DNA kit. Further analyses were based on the V3-V4 region of the 16S rRNA gene (Next Generation Sequencing method).
Results: We identified 8 clusters of bacteria (Table). The analysis revealed that the gut microbiome consists mostly of Bacteroidetes, Firmicutes and Actinobacteria, with the largest abundance of reads for Bacteroidetes (Table). HF-REF group was characterized with almost half of the operational taxonomic units reads in Firmicutes than group without HF-REF (Table). There was no difference in Firmicutes/Bacteroidetes ratio between study groups (Figure).
Conclusions: HF-REF among CAD patients was associated with altered microbiome composition. The analyses in larger subpopulations and in control group is required.