Purpose: To evaluate gut microbiota composition in CAD patients. To compare intestinal microbiome of patients with acute coronary syndrome (ACS) in relation to patients who underwent elective percutaneous coronary intervention (PCI).
Methods: The study included stool microbiome of 92 CAD patients, aged 64.6±7.6 years (72 males) who were hospitalized 12-18 months prior recruitment due to an ACS (ST-elevation myocardial infarction, n=22; non-ST elevation myocardial infarction, n=22; unstable angina, n=14) or PCI (n=34). All patients underwent detailed assessment of health status, including biochemical tests and stool sample collection.
Stool samples were collected in Stool Tubes with DNA stabilizer. DNA was isolated with the use of the PSP-Spin Stool-DNA kit. Further analyses were based on the V3-V4 region of the 16S rRNA gene (Next Generation Sequencing method).
Results: We identified 8 clusters of bacteria (Table). The analysis revealed that the gut microbiome consists mostly of Bacteroidetes, Firmicutes and Actinobacteria, with the largest abundance of reads for Bacteroidetes (Table). Study groups did not differ in terms of the most abundant bacteria (Table). There was no difference in Firmicutes/Bacteroidetes ratio between study groups (Figure).
Conclusions: Among CAD patients, there are no significant differences in gut microbiome composition between patients with ACS and PCI. The analysis in larger CAD population as well as patients without CAD is required.