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Proteostasis, inflammatory and vasoactive biomarkers in patients with hypertension: effect of exercise training

Session Poster Session 2

Speaker Manuel Teixeira

Event : ESC Preventive Cardiology (Formerly EuroPrevent) 2019

  • Topic : preventive cardiology
  • Sub-topic : Physical Inactivity and Exercise
  • Session type : Poster Session

Authors : M Teixeira (Aveiro,PT), M Gouveia (Aveiro,PT), A Duarte (Oliveira do Bairro,PT), M Ferreira (Oliveira do Bairro,PT), M Simoes (Oliveira do Bairro,PT), M Conceicao (Oliveira do Bairro,PT), G Silva (Oliveira do Bairro,PT), S Magalhaes (Aveiro,PT), R Ferreira (Aveiro,PT), A Nunes (Aveiro,PT), S I Vieira (Aveiro,PT), F Ribeiro (Aveiro,PT)

Authors:
M Teixeira1 , M Gouveia1 , A Duarte2 , M Ferreira2 , M Simoes2 , M Conceicao2 , G Silva3 , S Magalhaes4 , R Ferreira5 , A Nunes1 , S I Vieira1 , F Ribeiro6 , 1University of Aveiro, Department of Medical Sciences and Institute of Biomedicine – iBiMED - Aveiro - Portugal , 2Unidade Cuidados na Comunidade Cubo Mágico da Saúde - Oliveira do Bairro - Portugal , 3Câmara Municipal de Oliveira do Bairro - Oliveira do Bairro - Portugal , 4University of Aveiro, Department of Medical Sciences and iBiMED and Aveiro Institute of Materials – CICECO - Aveiro - Portugal , 5University of Aveiro, Department of Chemistry - Aveiro - Portugal , 6University of Aveiro, School of Health Sciences and Institute of Biomedicine – iBiMED - Aveiro - Portugal ,

Citation:

Background: Physical exercise is a consensual and well-established strategy to control blood pressure. Nonetheless, its effects on protein homeostasis in individuals with hypertension are not clearly defined.

Aims: This study aimed to evaluate the effects of regular exercise on inflammatory, oxidative stress and vasoactive markers in circulation, as well as protein homeostasis and quality of life in individuals with hypertension. Furthermore, we intended to correlate quality of life with the levels of the above-mentioned biomarkers.

Methods: A total of 20 individuals with hypertension were recruited and divided into 2 groups of 10: a group who regularly participated in physical exercise in the past year ("EH", age: 68.3 ± 4.2 years), and an age-matched group without regular exercise practice in the past year ("H", age: 67.7 ± 5.1 years). Clinical data, daily physical activity (IPAQ), quality of life (SF-36v2), adhesion to Mediterranean diet (MEDAS Questionnaire) and blood pressure were evaluated. Proteostasis and vasoactive markers were assessed in plasma using immunoblotting techniques (western blot or slot-blot) and Attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR). The levels of ubiquitin, Hsp70, IL-6, C-reactive protein, TWEAK, IL-10, eNOS, endothelin-1, MMP-2, TIMP-2, connexin 43 and superoxide dismutase (SOD) were analysed by western blot or slot-blot.

Results: Significant higher levels of eNOS (p=0.011), Cx43 (p=0.020), TIMP-2 (p=0.038) and SOD-3 (p=0.001), with a fold increase of 1.2, 2.1, 1.3 and 1.2, respectively, were found in the EH group. The overall quality of life (60.1 ± 4.3 vs. 53.2 ± 5.9, p=0.009), as well as mental health (59.4 ± 7.9 vs. 50.7 ± 7.2, p=0.024) was significantly higher in the EH group. A strong inverse correlation was observed between the quality of life (total score of the SF-36) and the levels of IL-6 (r=-0.510, p=0.026). The levels of IL-6 are significantly higher in the H group (p=0.014), with a 1.5-fold increase in comparison with the EH group, and partial least squares regression (PLS-R) analysis of FTIR data showed a high correlation between the levels of IL-6 and the spectra in 1500-900 cm-1 region. We found that high levels of IL-6 correlate with peaks associated to collagen and low levels correlate to peaks associated to carotenes. Principal component analysis (PCA) showed a good discrimination between the two groups in the 1500-900 cm-1 region (fingerprint region) and 1700-1600 cm-1 region (Amide I band of proteins). Furthermore, the H group showed peaks that are related with anti-parallel ß-sheet, while EH group showed peaks related to ß-sheet and a-helix.

Conclusion: Regular exercise participation reduced the circulating levels of the inflammatory biomarkers, increased the antioxidant defences and the levels of the vasodilator biomarkers and improved proteostasis and quality of life in individuals with hypertension.

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