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Influence of intensity of dynamic training on HSP70 expression by peripheral blood mononuclear cells from patients with chronic heart failure: a randomized controlled trial

Session Poster Session 7

Speaker Anderson Ulbrich

Event : ESC Congress 2013

  • Topic : preventive cardiology
  • Sub-topic : Rehabilitation: Outcomes
  • Session type : Poster Session

Authors : A Zampier Ulbrich (Florianopolis,BR), VG Angarten (Florianopolis,BR), SW Sties (Florianopolis,BR), A Schmitt Netto (Florianopolis,BR), LS Mara (Florianopolis,BR), A Bittencourt (Porto Alegre,BR), TG Heck (Ijuí,BR), PIH Bittencourt (Porto Alegre,BR), EL Silva (Florianopolis,BR), T Carvalho (Florianopolis,BR)

A. Zampier Ulbrich1 , V.G. Angarten1 , S.W. Sties1 , A. Schmitt Netto1 , L.S. Mara1 , A. Bittencourt2 , T.G. Heck3 , P.I.H. Bittencourt2 , E.L. Silva4 , T. Carvalho1 , 1State University of Santa Catarina - Florianopolis - Brazil , 2Federal University of Rio Grande do Sul (UFRGS) - Porto Alegre - Brazil , 3Regional University of Northwestern Rio Grande do Sul - Ijuí - Brazil , 4Federal University of Santa Catarina - Florianopolis - Brazil ,

On behalf: Center for Cardiology and Exercise Medicine

European Heart Journal ( 2013 ) 34 ( Abstract Supplement ), 1081-1082

Background: It has been suggested that the heat shock proteins (HSP70) plays a cardioprotective role against stress observed in chronic heart failure (CHF). Exercise is a strong inducer of HSP70 expression in cells from various tissues, that is associated with immune responses and intracellular cytoprotection in peripheral blood mononuclear cells (BMC). Objective: To determine the effects of different intensities of treadmill training on HSP70 expression in BMC from patients with CHF.

Methods: 22 patients with CHF, males (45 to 72 years), NYHA functional class II and III, were randomized into two groups to carry out a program of exercise at different intensities: moderate intensity training (MIT) and high intensity training (HIT), underwent three months of treadmill exercise (40 min, 3 times/week). BMC were collected at the beginning and the end of 12 weeks, before and after training session (TS). After, cells were subdivided into two extra stress groups to be cultivated at normal temperature (37°C) or submitted to heat shock (HS) at 42 °C for 2h. Then, the cells remained for an additional 6h recovery period at 37 °C for the further analysis of intracellular HSP70 by Western blot (SDS-PAGE).

Results: HSP70 expression increased after MIT and HIT training both before and after performing exercise (p<0.01). When the cells were submitted to the second challenge (HS), the same profile was observed in MIT group in all situations whereas in HIT group a significant increase was observed in heat-shocked cells only after the last exercise session of the training when compared with the first session (Fig. 1).

Conclusion: The immune response and the cytoprotection varied with the intensity of exercise. The MIT caused the best response on HSP70 expression by peripheral BMC from patients with CHF.

Figure 1. Effects of intensity on HSP70 in CHF

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