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2-AG impacts on endothelial cell activation and endothelial cell viability in vitro and impairs endothelial repair in vivo

Session Poster Session 1

Speaker Julian Jehle

Event : ESC Congress 2019

  • Topic : basic science
  • Sub-topic : Basic Science - Vascular Biology and Physiology
  • Session type : Poster Session

Authors : J Jehle (Bonn,DE), M Danisch (Bonn,DE), S Bagheri (Bonn,DE), E Avraamidou (Bonn,DE), V Tiyerili (Bonn,DE), P Pfeifer (Bonn,DE), L Bindila (Mainz,DE), B Lutz (Mainz,DE), A Zimmer (Bonn,DE), G Nickenig (Bonn,DE)

Authors:
J. Jehle1 , M. Danisch1 , S. Bagheri1 , E. Avraamidou1 , V. Tiyerili1 , P. Pfeifer1 , L. Bindila2 , B. Lutz2 , A. Zimmer3 , G. Nickenig1 , 1University Hospital Bonn, Department of Cardiology - Bonn - Germany , 2Johannes Gutenberg University Mainz (JGU) - Mainz - Germany , 3University Hospital Bonn - Bonn - Germany ,

Citation:
European Heart Journal ( 2019 ) 40 ( Supplement ), 321

Background: The endocannabinoid (eCB) 2-arachidonoylglycerol (2-AG) is a known modulator of inflammation and few studies have addressed its influence on myeloid cells in the context of atherogenesis. However, the impact of 2-AG on endothelial cell function has not been studied before.

Methods: Endothelial repair was studied in two treatment groups of wildtype mice following electrical denudation of the common carotid artery at a length of 3000 μm. One group received the monoacylglycerol lipase (MAGL)-inhibitor JZL184 [5 mg/kg i.p.], which impairs 2-AG degradation and thus causes elevated 2-AG levels, the other group received vehicle. The residual endothelial gap at five days in either group was visualized by Evan's blue staining. In vitro, the effect of 2-AG on human coronary artery endothelial cell (HCAEC) viability was assessed by an XTT-based assay. Endothelial activation was studied by an adhesion assay of THP-1 monocytes to 2-AG-preconditioned HCAEC. HCAEC migration, ROS-production, expression of NADPH oxidases, and secretion of inflammatory cytokines were assessed by Boyden chamber, qPCR, and colorimetric assays.

Results: Treatment with JZL184 produced a significant increase in 2-AG levels and impaired reendothelialisation in wildtype mice following electrical injury of the common carotid artery. The residual denudation at 5 days yielded 2291±286 μm in JZL184-treated animals vs. 1505±223 μm in vehicle treated controls (n=18–19; p<0.05). In vitro, JZL184 significantly reduced viability of HCAEC at 24 hours (0.31±0.10 vs. 1.00±0.08; n=3; p<0.01). Finally, 2-AG promoted HCAEC activation resulting in a significant increase in THP-1 monocyte adhesion to HCAEC following pre-treatment of HCAEC with 2-AG (0.17±0.03 THP-1 cells per HCAEC vs. 0.07±0.01 THP-1 cells per HCAEC; n=3; p<0.05). Besides, HCAEC migration, ROS-production, expression of NADPH oxidases and secretion of inflammatory cytokines were unaffected by 2-AG.

Conclusion: Elevated 2-AG levels appear to hamper endothelial repair and to promote HCAEC activation and cell death. Our data suggest that besides its influence on myeloid cells, 2-AG is also adverse to endothelial integrity which might promote early atherosclerotic lesion formation. Thus, decreasing vascular 2-AG levels might represent a promising therapeutic strategy for the prevention of atherosclerosis and coronary heart disease.

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