Purpose: Adipose tissue is an inflammatory source of coronary artery disease (CAD). Kinin receptors may be an important determinant of the impact of adiposity on endothelial function and cardiovascular function. The aim of this study was to examine kinin receptors gene expression levels in the adipose tissue in obese patients with and without CAD.
Methods: Obese patients (BMI >30kg/m2) undergoing coronary angiogram were participated in the study. We included 15 patients with CAD (9 male, 64±8 years old) and 9 patients without CAD (6 male, 63±8 years old). Subcutaneous white adipose tissue biopsies were obtained from the site of paracentesis before the procedure and analyzed for bradykinin receptor type 1 (BR1) and 2 (BR2) gene expression by RT-quantitative PCR.
Results: Adipose tissue analysis revealed increased BR1 and BR2 gene expression levels in obese patients with CAD compared to those without CAD. More specifically, BR1 gene expression levels were 654±345 in patients with CAD versus 107±99 in patients without CAD and BR2 gene expression levels were 458±165 in patients with CAD versus 133±107 in patients without CAD (p<0.05 for both). No statistical significant correlation were found between BR1 and BR2 gene expression levels and patients' BMI.
Conclusions: There is a divergence in BR1 and BR2 gene expression in adipose tissue between obese patients who exhibit or do not exhibit CAD. Our findings may have implications in the pathophysiology and treatment of atherosclerosis and should be further investigated.