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Exercise training leads to an increase of the proangiogenic TIE2 monocyte/ EPC ratio in patients with peripheral arterial disease over 6 months

Session Poster session 3

Speaker Joern Fredrik Dopheide

Event : ESC Congress 2015

  • Topic : preventive cardiology
  • Sub-topic : Exercise Testing
  • Session type : Poster Session

Authors : J F Dopheide (Mainz,DE), J Rubrech (Mainz,DE), A Trumpp (Mainz,DE), P Geissler (Mainz,DE), T Gori (Mainz,DE), M P Radsak (Mainz,DE), T Muenzel (Mainz,DE), C Espinola-Klein (Mainz,DE)

J.F. Dopheide1 , J. Rubrech1 , A. Trumpp1 , P. Geissler1 , T. Gori1 , M.P. Radsak1 , T. Muenzel1 , C. Espinola-Klein1 , 1University Hospital of Mainz - Mainz - Germany ,

European Heart Journal ( 2015 ) 36 ( Abstract Supplement ), 460

Background: Tie2+ monocytes (TEM) and endothelial progenitor cells (EPC) play a crucial role in neoangiogenesis. In peripheral arterial disease (PAD) exercise training can promote angiogenesis and thus ameliorate the severity of the disease.

Methods: 40 PAD patients with intermittent claudicatio (IC, Fontaine State II a and b) were asked to perform either a supervised (SET (n=20)) or a non-supervised exercise training (NET (n=20)). Peripheral blood leukocytes were analysed from whole blood by flow cytometry (Beckman-Coulter Navios 10/3). Monocytes and EPC were identified by different gating strategies in relation to size and granulation (FSC/ SSC) and surface molecules (CD14/CD86/CD45/CD34) and analysed for CD14/CD16/Tie2 or CD34+/VEGF-R2+/CD45-, respectively. The results were compared with an age matched control group (n=20).

Results: At admission patients in total showed an increased proportion of EPC and reduced proportion of TEM (both p<0.001) in comparison to controls, leading to a TEM/ EPC ratio in favour of EPC (p<0.0001). After 6 months training, we observed a decrease in EPC proportion for patients (p<0.01) with a shift of the TEM/ EPC ratio in favour of TEM (p<0.01), leading to no difference between patients and controls in regard to EPC and their ratio. TEM proportions though were still lower for patients (p<0.05). Comparison of SET vs. NET showed a higher proportion of TEM for the SET group, and thus an increased TEM/ EPC ratio (both p<0.05). The absolute walking distance in the SET group was higher than in NET (725±457 m vs. 545±192 m) though not significant (p=0.92).

Conclusions: Exercise training can influence the distribution of proangiogenic cells. The depletion of EPC from the circulatory pool might be related to a potentially increased collateral growth, leading to a shift of the TEM/EPC ratio in favour of TEMs. SET seems to be more effective in regard to TEM than NET. Thus, the TEM/ EPC ratio might be used as a helpful tool to monitor the effectiveness of any type of exercise training.

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